The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric area under curve method for estimation of pioglitazone hydrochloride. This area under curve of UV spectrum between 221 to 231 nm was validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 10-50 μg/ml. The value of correlation coefficient was 0.999. Satisfactory values of percent relative standard deviation for the intra-day and inter-day precision indicated that method was precise. Results of the recovery studies (96.62% to 108.84%) showed accuracy of the method. LOD and LOQ were calculated as 0.16596 μg/ml and 0.50292 μg/ml, respectively. The developed method can be used for routine estimation of pioglitazone hydrochloride in bulk and tablet dosage forms.
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